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Related Experiment Videos

Antipneumococcal activity of telithromycin by agar dilution, microdilution, E test, and disk diffusion methodologies.

T A Davies1, L M Kelly, M R Jacobs

  • 1Department of Pathology, Hershey Medical Center, Hershey, PA 17033, USA.

Journal of Clinical Microbiology
|April 4, 2000
PubMed
Summary

Telithromycin demonstrates high activity against both erythromycin-susceptible and resistant pneumococci, regardless of resistance mechanism. Susceptibility testing using various methods confirmed telithromycin

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Area of Science:

  • Microbiology
  • Pharmacology
  • Infectious Diseases

Background:

  • Erythromycin resistance in Streptococcus pneumoniae is a growing public health concern.
  • Telithromycin, a ketolide antibiotic, exhibits broad-spectrum activity.
  • Evaluating telithromycin's efficacy against resistant pneumococcal strains is crucial for clinical application.

Purpose of the Study:

  • To assess the in vitro activity of telithromycin against erythromycin-susceptible and -resistant pneumococcal isolates.
  • To compare the performance of different antimicrobial susceptibility testing methods for telithromycin.

Main Methods:

  • Agar dilution, microdilution, E test, and disk diffusion were employed.
  • Testing was performed on 110 erythromycin-susceptible and 106 erythromycin-resistant pneumococcal strains.

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  • Incubation conditions included both air and 2% CO(2).

    • Main Results:

    • Telithromycin exhibited potent activity (MIC(50)/MIC(90) ranging from 0.008/0.016 to 0.125/0.5 µg/ml) against all tested pneumococcal strains.
    • Essential agreement between methods varied, with E test in air showing 77.8% agreement with agar dilution.
    • Over 99% of strains were susceptible to telithromycin at a breakpoint of ≤1 µg/ml, irrespective of incubation conditions or resistance mechanism.

    Conclusions:

    • Telithromycin is highly effective against erythromycin-susceptible and -resistant pneumococci, including those with erm and mef determinants.
    • Agar dilution, microdilution, E test, and disk diffusion are reliable methods for telithromycin susceptibility testing.
    • Standardized breakpoints ensure accurate susceptibility determination, with incubation conditions influencing zone sizes and MICs.