Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microarray fabrication with covalent attachment of DNA using bubble jet technology.

T Okamoto1, T Suzuki, N Yamamoto

  • 1Canon Research Center, 5-1, Morinosato-Wakamiya, Atsugi-shi, Kanagawa, 243-0193, Japan.

Nature Biotechnology
|April 5, 2000
PubMed
Summary

Researchers created a novel DNA microarray fabrication method using inkjet technology to detect mutations in the p53 gene. This method accurately identifies single-base mismatches in DNA, crucial for cancer research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoreaction of N560 intermediate in the photocycle of bacteriorhodopsin.

FEBS letters·1992
Same author

Mechanism of anti-human immunodeficiency virus action of polyoxometalates, a class of broad-spectrum antiviral agents.

Molecular pharmacology·1992
Same author

Cell type-specific anti-human immunodeficiency virus type 1 activity of the transactivation inhibitor Ro5-3335.

Antimicrobial agents and chemotherapy·1992
Same author

Regulation of differentiation and proliferation of rat hepatocytes by lactose-carrying polystyrene.

Artificial organs·1992
Same author

Inhibitory effect of tannic acid sulfate and related sulfates on infectivity, cytopathic effect, and giant cell formation of human immunodeficiency virus.

Planta medica·1992
Same author

[Coronary revascularization with bilateral internal thoracic artery grafts].

[Zasshi] [Journal]. Nihon Kyobu Geka Gakkai·1992

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • The p53 tumor-suppressor gene is critical in preventing cancer.
  • Accurate detection of mutations in the p53 gene is essential for cancer diagnosis and research.
  • Current methods for DNA mutation detection can be labor-intensive and require specialized equipment.

Purpose of the Study:

  • To develop a novel, high-throughput method for fabricating DNA microarrays.
  • To create DNA microarrays capable of detecting specific mutations in the p53 gene.
  • To validate the sensitivity and specificity of the fabricated DNA microarrays for mutation detection.

Main Methods:

  • Utilized a Bubble Jet ink jet device for precise deposition of 5'-terminal-thiolated oligonucleotides onto a glass surface.

Related Experiment Videos

  • Employed heterobifunctional crosslinkers for stable covalent attachment of oligonucleotides.
  • Fabricated DNA microarrays containing 64 groups of 18-mer oligonucleotides targeting all three-base mutations in the p53 gene's mutational hot spot.

    • Main Results:

    • Successfully fabricated DNA microarrays with high probe density and specificity.
    • Demonstrated the ability to screen microarrays using fluorescently labeled synthetic oligonucleotides and PCR-amplified p53 gene segments.
    • Achieved accurate discrimination between perfectly matched hybrids and those with single-base mismatches.

    Conclusions:

    • The developed inkjet-based DNA microarray fabrication method is efficient and reliable.
    • This technology enables sensitive and specific detection of p53 gene mutations.
    • The method holds significant potential for applications in cancer diagnostics and molecular screening.