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Related Experiment Videos

Mesenteric dysfunction after cardiopulmonary bypass: role of complement C5a.

M Tofukuji1, G L Stahl, C Metais

  • 1Department of Surgery, Beth Israel-Deaconess Medical Center, Boston, Massachusetts 02215, USA.

The Annals of Thoracic Surgery
|April 6, 2000
PubMed
Summary

Cardiopulmonary bypass (CPB) causes ileal injury. Inhibiting complement C5a improves microvascular regulation but not overall mesenteric dysfunction after CPB.

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Area of Science:

  • Gastroenterology
  • Immunology
  • Cardiovascular Surgery

Background:

  • Cardiopulmonary bypass (CPB) can induce mesenteric injury.
  • The role of complement C5a in CPB-induced ileal damage is not fully understood.

Purpose of the Study:

  • To investigate the effects of CPB on ileal homeostasis.
  • To determine if inhibiting complement C5a can mitigate CPB-induced mesenteric injury.

Main Methods:

  • Pigs underwent 1 hour of CPB followed by 2 hours off CPB.
  • An anti-C5a monoclonal antibody (C5a MAb) was administered pre-CPB to inhibit C5a.
  • Measurements included complement activity, ileal myeloperoxidase, and ileal integrity.

Main Results:

  • C5a MAb treatment improved microvessel function (relaxation to ADP/substance P, reduced hypercontractility to phenylephrine).

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  • Ileal myeloperoxidase activity was significantly lower in the C5a MAb group compared to controls.
  • Despite microvascular improvements, mucosal blood flow, pH, edema, and permeability changes were similar between groups.

    • Conclusions:

    • CPB causes significant physiological alterations in the ileum, affecting perfusion, permeability, and blood flow regulation.
    • C5a inhibition partially protects against CPB-induced ileal microvascular dysfunction by limiting neutrophil activity.
    • Inhibition of C5a does not prevent extravascular mesenteric dysfunction following CPB.