Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basic fibroblast growth factor positively regulates hematopoietic development.

P Faloon1, E Arentson, A Kazarov

  • 1Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.

Development (Cambridge, England)
|April 6, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutron-Multiplicity Measurement in Muon Capture on Oxygen Nuclei in the Gadolinium-Loaded Super-Kamiokande Detector.

Physical review letters·2026
Same author

Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

Physical review letters·2026
Same author

Sleep disturbances in patients with myasthenia gravis: A cross-sectional study.

Neurologia·2026
Same author

Evidence for the Dimuon Decay of the Higgs Boson in pp Collisions with the ATLAS Detector.

Physical review letters·2025
Same author

[Analysis of peripheral blood monocyte subsets in chronic myelomonocytic leukemia: a single-center study].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

Evidence for Longitudinally Polarized W Bosons in the Electroweak Production of Same-Sign W Boson Pairs in Association with Two Jets in pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

Physical review letters·2025

Newly found BLast Colony Forming Cells (BL-CFCs) are key to understanding hematopoietic commitment. Research shows fibroblast growth factor receptor-1 (FGFR1) signaling is vital for hemangioblast proliferation and development.

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Hematopoiesis

Background:

  • BLAST Colony Forming Cells (BL-CFCs) derived from embryonic stem (ES) cells are the hemangioblast, a common progenitor for hematopoietic and endothelial cells.
  • Studying BL-CFCs offers a unique window into the early events of hematopoietic commitment.

Purpose of the Study:

  • To quantitatively assess the impact of mesoderm-inducing factors on hematopoietic lineage development using BL-CFC assays and Flk1(+) cell analysis.
  • To elucidate the molecular events governing hemangioblast development by analyzing ES cell lines with targeted mutations in FGFR1 and SCL.

Main Methods:

  • Utilized BL-CFC assays and fluorescent activated cell sorter (FACS) analysis for Flk1(+) cells.
  • Investigated ES cell lines with targeted mutations in fibroblast growth factor receptor-1 (FGFR1) and the transcription factor SCL.

Related Experiment Videos

Main Results:

  • BL-CFCs express the receptor tyrosine kinase Flk1.
  • Fibroblast growth factor (FGF) signaling, particularly through FGFR1, is crucial for hemangioblast proliferation.
  • Cells co-expressing Flk1 and SCL are proposed to represent the hemangioblast population.

Conclusions:

  • Basic fibroblast growth factor (bFGF)-mediated signaling plays a critical role in hemangioblast proliferation.
  • The combined expression of Flk1 and SCL may define the hemangioblast, providing a molecular marker for this progenitor cell population.