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Related Concept Videos

Glial Cells01:04

Glial Cells

Overview
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When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
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Related Experiment Video

Updated: Jul 15, 2026

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
07:27

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

Published on: January 31, 2019

ATP-mediated glia signaling.

M L Cotrina1, J H Lin, J C López-García

  • 1Department of Cell Biology and Anatomy, New York Medical College, Valhalla, New York 10595, USA.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|February 7, 2001
PubMed
Summary

Glia calcium waves, driven by ATP and purinergic receptors, expand spatially. Connexin expression enhances ATP release and neuron growth, highlighting ATP

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Glia calcium signaling modulates synaptic transmission.
  • The mechanisms of glia calcium wave propagation are under investigation.

Purpose of the Study:

  • To elucidate the role of ATP and purinergic receptors in glia calcium wave expansion.
  • To investigate the impact of connexin expression on glia function and neuronal development.

Main Methods:

  • Studied spatial expansion of calcium waves in glia.
  • Investigated the role of ATP and purinergic receptors.
  • Examined the effects of connexin (Cx) expression on ATP release and calcium wave propagation.
  • Co-cultured cortical neurons with Cx-expressing and Cx-deficient glia.
  • Utilized purinergic receptor antagonists.

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Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Published on: December 19, 2019

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
11:36

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

Related Experiment Videos

Last Updated: Jul 15, 2026

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
07:27

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

Published on: January 31, 2019

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
11:20

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Published on: December 19, 2019

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
11:36

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

Main Results:

  • Spatial expansion of glia calcium waves is mediated by ATP and purinergic receptor activation.
  • Ectopic connexin expression increased ATP release and calcium wave propagation radius.
  • Connexin expression induced a phenotypic transformation in glia, promoting neurite outgrowth in co-cultured neurons.
  • Purinergic receptor antagonists reversed glia phenotypic changes and slowed neurite outgrowth.

Conclusions:

  • ATP plays a crucial role in both short-term glia calcium signaling and long-term neuronal differentiation.
  • Connexins and purinergic signaling are key regulators of glia-neuron interactions and neuronal development.