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[Information behavior of microsatellite loci in genome scanning]
1Molecular Medical Center, Ruijin Hospital, Shanghai Second Medical University, Shanghai, 200025 P. R. China.
Abstract
Objective:
To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population.
Methods:
Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan( TM) and Genotyper(TM).
Results:
The heterozygosities are between 0.35 to 0.89, with the heterozygosities of 88% loci >0.60. PICs are in the range of 0.32 and 0.88, with PICs of 95% loci >0.50.
Conclusion:
The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.