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PPMdb: a plant plasma membrane database.

I Sahnoun1, P Déhais, M Van Montagu

  • 1Department of Plant Genetics, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000, Gent, Belgium.

Journal of Biotechnology
|April 7, 2000
PubMed
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PPMdb is a new proteome database for plant plasma membrane proteins, offering 2-D PAGE maps, sequences, and expression data. This resource aids researchers in plant proteomics and cell biology.

Area of Science:

  • Proteomics
  • Plant Biology
  • Bioinformatics

Background:

  • Plant plasma membrane proteins are crucial for cellular functions.
  • Comprehensive databases are needed to organize and access proteomic data.

Purpose of the Study:

  • To introduce PPMdb, a dedicated database for plant plasma membrane proteins.
  • To provide a centralized resource for proteomic information, including 2-D PAGE maps, sequences, and expression data.

Main Methods:

  • Data collection and annotation of plant plasma membrane proteins.
  • Structuring information into a relational database.
  • Integration with external biological databases (EMBL, GenBank, SWISS-PROT, etc.) and MEDLINE.

Main Results:

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  • PPMdb offers comprehensive 2-D PAGE maps, partial amino acid sequences, and expression data.
  • The database allows users to query by accession number, protein name, pI, MW, and cellular location.
  • Interactive display of protein spots by clicking on 2-D maps.

Conclusions:

  • PPMdb serves as a valuable resource for plant proteomics research.
  • Facilitates access to integrated proteomic and expression data for plant plasma membrane proteins.