Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Green fluorescent protein as a quantitative tool.

N J Hack1, B Billups, P B Guthrie

  • 1Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City 84132, USA.

Journal of Neuroscience Methods
|April 7, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of an immune-evading doxycycline-inducible lentiviral vector for gene therapy in the spinal cord.

Experimental neurology·2022
Same author

Concurrent advanced HIV disease and viral load suppression in a high-burden setting: Findings from the 2015-6 ZIMPHIA survey.

PloS one·2020
Same author

On the Treatment of Giardiasis in Rats with Arsenobenzol.

The Journal of medical research·2009
Same author

Functional expression of two system A glutamine transporter isoforms in rat auditory brainstem neurons.

Neuroscience·2009
Same author

Detecting synaptic connections in the medial nucleus of the trapezoid body using calcium imaging.

Pflugers Archiv : European journal of physiology·2002
Same author

Matrix metalloproteases and their inhibitors are produced by overlapping populations of activated astrocytes.

Brain research. Molecular brain research·2002
Same journal

Pupil-DLC: an open-source deep learning pipeline for scalable, marker-less tracking of pupil dynamics across conscious and unconscious states.

Journal of neuroscience methods·2026
Same journal

Time as the language of Behavior: events, sequences, patterns and meanings.

Journal of neuroscience methods·2026
Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026
Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026
Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026
Same journal

Methods for measuring neural activity during voluntary wheel running.

Journal of neuroscience methods·2026
See all related articles

Researchers developed a simple method to quantify protein expression in single cells using green fluorescent protein (GFP) tags. This technique revealed a link between calretinin (CR) protein levels and cellular calcium handling.

Area of Science:

  • Cell biology
  • Biochemistry
  • Neuroscience

Background:

  • Understanding protein function requires measuring physiologically relevant concentrations.
  • Current methods may lack precision for quantifying protein expression in single cells.

Purpose of the Study:

  • To develop a simple, quantitative method for measuring overexpressed protein concentrations in single cells.
  • To assess the relationship between protein expression levels and physiological properties.

Main Methods:

  • Transfection of teratocarcinoma cells with calretinin (CR) fused to green fluorescent protein (GFP).
  • Quantification of CR-GFP levels using a standard curve derived from GFP fluorescence measurements.
  • Assessment of cellular calcium clearance capacity using Fura-2 imaging in the same cells.

Related Experiment Videos

Main Results:

  • Established a standard curve for GFP fluorescence to quantify CR-GFP concentrations in individual cells.
  • Demonstrated a strong positive correlation between CR-GFP expression and calcium clearance capacity.
  • Validated the utility of GFP fluorescence for quantitative protein expression analysis.

Conclusions:

  • The developed method allows reliable quantification of GFP-tagged fusion protein expression in single cells.
  • This approach enables the study of covariation between protein expression and cellular functions.
  • Provides a valuable tool for functional studies of proteins at physiologically significant levels.