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Updated: Jun 28, 2026
09:53
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
Published on: January 1, 2018
Protecting the Inner Ear from Acoustic Trauma.
The International Tinnitus Journal
|February 7, 2001
Summary
Leupeptin, a calpain inhibitor, showed potential in protecting against noise-induced hearing loss. Treated ears experienced less hearing impairment and hair cell damage compared to control ears after noise exposure.
Area of Science:
- Neuroscience
- Otolaryngology
- Pharmacology
Background:
- Calpains (calcium-activated proteases) are implicated in neurodegeneration.
- Leupeptin, a calpain inhibitor, can reduce neural degeneration.
- Noise-induced hearing loss and associated hair cell damage are significant concerns.
Purpose of the Study:
- To investigate the protective effects of leupeptin against noise-induced hearing loss.
- To assess leupeptin's efficacy in preventing cochlear hair cell loss.
Main Methods:
- Leupeptin was infused into the scala tympani of one cochlea.
- A 14-day exposure to 100 dB SPL octave band noise (4.0 kHz center) was administered.
- Auditory evoked response was used to assess hearing loss.
- Hair cell loss was evaluated post-exposure.
Main Results:
- Leupeptin-treated ears showed reduced hearing loss during early recovery.
- Significantly less hair cell loss was observed in leupeptin-treated ears.
- Preliminary data indicate a protective effect of leupeptin.
Conclusions:
- Leupeptin may offer protection against noise-induced hearing loss.
- Calpain inhibition could be a therapeutic strategy for preventing noise-induced cochlear damage.
Related Concept Videos
01:13The Cochlea
The cochlea is a coiled structure in the inner ear that contains hair cells—the sensory receptors of the auditory system. Sound waves are transmitted to the cochlea by small bones attached to the eardrum called the ossicles, which vibrate the oval window that leads to the inner ear. This causes fluid in the chambers of the cochlea to move, vibrating the basilar membrane.
01:11The Auditory Ossicles
The auditory ossicles of the middle ear transmit sounds from the air as vibrations to the fluid-filled cochlea. The auditory ossicles consist of two malleus (hammer) bones, two incus (anvil) bones, and two stapes (stirrups), one on each side. These bones develop during the fetal stage and are the ones to ossify first. They are fully mature at birth and do not grow afterward.
The aptly named stapes look very much like a stirrup. The three ossicles are unique to mammals, and each plays a role in...
The aptly named stapes look very much like a stirrup. The three ossicles are unique to mammals, and each plays a role in...
01:16Anatomy of the Ear
Auditory sensation, commonly called hearing, involves the transformation of sonic waves into neural impulses facilitated by the structures of the auditory organ. The prominent, flesh-like structure on the side of the head, called the auricle, directs sound waves towards the auditory canal. The auricle is often mislabeled as the pinna, a term more aligned with mobile structures like a feline's external ear. The auditory canal penetrates the cranium via the external auditory meatus of the...
01:15Auditory Pathway
Auditory pathways constitute the complex neural circuits responsible for transmitting and interpreting auditory information from the peripheral auditory system to the brain. Sound waves are initially captured by the outer ear, funneled through the ear canal, and reach the tympanic membrane (eardrum). These vibrations are transmitted via the middle ear's ossicles to the inner ear's cochlea.
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking the...
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking the...
01:15Equilibrium and Balance
The inner ear assumes dual functionalities of auditory perception and equilibrium maintenance. The vestibule is the organ responsible for balance. This organ contains mechanoreceptors, specifically hair cells, endowed with stereocilia, which aid in deciphering information regarding the position and motion of our heads. Two intrinsic components, the utricle and saccule, help perceive head position, while the semicircular canals track head movement. Neurological messages initiated in the...
01:17Auditory Perception
The auditory system is essential for sound perception, utilizing various critical structures. When sound waves enter the outer ear, they travel through the ear canal and cause the eardrum to vibrate. These vibrations are then transmitted to the middle ear, where three tiny bones – the malleus, incus, and stapes – amplify the sound. This amplification is crucial, as it ensures that the sound vibrations are strong enough to be conveyed to the inner ear. These vibrations then reach the cochlea, a...