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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
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High-Resolution Absorption Spectroscopy of the 3nu(1) and 3nu(1) + nu(3) Bands of Propyne

Campargue1, Bertseva, Graner

  • 1Laboratoire de Spectrométrie Physique (associated with CNRS UMR C5588), Université Joseph Fourier de Grenoble, Saint-Martin-d'Hères Cedex, 38402, France

Journal of Molecular Spectroscopy
|February 7, 2001
PubMed

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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