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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
Identification of regulatory regions of the putative tumor suppressor gene DMBT1
E Lualdi1, E Chiariello, G Finocchiaro
1Unit of Neuro-Oncology and Gene Therapy, Istituto Nazionale Neurologico Besta, via Celoria 11, Milan, 20133, Italy.
Abstract:
The lack of expression of the putative tumor suppressor gene DMBT1 has been described in gliomas, lung carcinomas, and other malignancies. To investigate the mechanisms regulating DMBT1 expression we have screened a human genomic library with a 5' cDNA probe and identified a fragment of approximately 3 kb upstream of the proposed coding sequence of DMBT1. This fragment contains subregions that may up- or down-regulate the expression of a reporter gene. These findings may help to elucidate the mechanisms regulating the alternative splicing and the tissue specific expression of DMBT1.
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