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Preservation of 5'-end integrity of a potyvirus genomic RNA is not dependent on template specificity
L Simón-Buela1, L Osaba, J A García
1Centro Nacional de Biotecnología, CSIC, Campus UAM, Madrid, 28049, Spain.
Virology
|February 7, 2001
Abstract:
Full-length in vitro transcripts of plum pox potyvirus (PPV) genomic RNA with mutations altering the number of 5'-terminal adenosine residues were able to infect Nicotiana clevelandii plants, whereas a mutant with a substitution of adenosine in position 2 by guanosine failed to infect. The genomic 5' end was template-independently repaired during in vivo RNA synthesis producing wild-type viral progeny. Putative models of replication initiation are discussed.