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Study of the in vitro maturation of mouse oocytes induced by microinjection of maturation promoting factor (MPF)
1First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan.
Purpose:
Maturation promoting factor (MPF) acts at the resumption of meiosis and nonspecifically throughout the animal species. There exists a considerable body of literature on MPF, but little work has been done to study the induction of maturation of mammalian oocytes by microinjection of extracted MPF.
Methods:
Immature (GV-stage) mouse oocytes were microinjected MPF extracted from matured Xenopus eggs in the presence of dbcAMP.
Results:
The rate of germinal vesicle, breakdown (GVBD) induced at 24 hr after MPF injection was significantly higher (90.5%) than that of the control (2.2%), which was injected with HTF medium containing dbcAMP (P < 0.0001). The rate of extrusion of the first polar body at 24 hr after MPF injection was significantly higher (84.1%) than that of the same control (1.1%) (P < 0.0001).
Conclusions:
From these results, it is concluded that the maturation of mammalian oocytes can be induced by the microinjection of MPF extracted from other species.
Insights
Microinjection of maturation promoting factor (MPF) from Xenopus eggs successfully induced oocyte maturation in mice. This study demonstrates cross-species efficacy for MPF in promoting meiosis resumption and polar body extrusion.
Area of Science:
- Developmental Biology
- Cell Biology
- Reproductive Biology
Background:
- Maturation promoting factor (MPF) is crucial for meiosis resumption across animal species.
- While MPF's role is well-documented, its application in inducing mammalian oocyte maturation via microinjection remains underexplored.
Purpose of the Study:
- To investigate the efficacy of microinjecting MPF extracted from Xenopus eggs to induce maturation in mammalian oocytes.
- To determine if cross-species MPF can overcome species-specific barriers in oocyte maturation.
Main Methods:
- Immature mouse oocytes at the germinal vesicle (GV) stage were microinjected with MPF extracted from mature Xenopus eggs.
- Oocytes were cultured in the presence of dbcAMP, with controls receiving HTF medium containing dbcAMP.
Main Results:
- Microinjection of MPF significantly increased the rate of germinal vesicle breakdown (GVBD) to 90.5% compared to 2.2% in controls (P < 0.0001).
- The rate of first polar body extrusion was also significantly higher in MPF-injected oocytes (84.1%) versus controls (1.1%) (P < 0.0001).
Conclusions:
- Microinjection of MPF from one species can effectively induce maturation in mammalian oocytes.
- This finding supports the conserved role of MPF in oocyte maturation and suggests potential applications in assisted reproduction.