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Related Experiment Videos

Subgenomic negative-strand RNA function during mouse hepatitis virus infection.

R S Baric1, B Yount

  • 1Department of Epidemiology, Program in Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA. rbaric@sph.unc.edu

Journal of Virology
|February 7, 2001
PubMed
Summary

Subgenomic negative-strand RNAs are key templates for synthesizing positive-strand RNA in Mouse hepatitis virus (MHV) infections. These findings clarify the role of these viral RNAs in MHV replication.

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Area of Science:

  • Virology
  • Molecular Biology
  • RNA Biology

Background:

  • Mouse hepatitis virus (MHV) infection involves both full-length and subgenomic RNA strands.
  • The precise origin and function of subgenomic negative-strand RNAs in MHV replication remain debated.

Purpose of the Study:

  • To investigate the role of subgenomic negative-strand RNAs in the synthesis of positive-strand RNA during MHV infection.
  • To determine if subgenomic negative-strand RNAs serve as principal templates for positive-strand RNA synthesis.

Main Methods:

  • Comparative analysis of RNA synthesis in alternative host cells.
  • Kinetic labeling experiments to track RNA accumulation and synthesis rates.
  • Cycloheximide treatment to assess the impact on RNA synthesis and transcription complexes.

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Main Results:

  • Subgenomic negative-strand RNA synthesis and molar ratios are consistent across different host cells.
  • Kinetic labeling indicates subgenomic negative-strand RNAs are rapidly synthesized and are principal mediators of positive-strand synthesis.
  • Cycloheximide inhibits both full-length and subgenomic negative-strand synthesis, with pre-existing negative strands remaining active.

Conclusions:

  • Subgenomic negative-strand RNAs are the primary templates for positive-strand RNA synthesis in MHV-infected cells.
  • These findings resolve the controversy surrounding the function of subgenomic negative-strand RNAs in MHV replication.