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Novel simulated moving-bed method for reduced solvent consumption
T B Jensen1, T G Reijns, H A Billiet
1Kluyver Laboratory for Biotechnology, Delft University of Technology, The Netherlands.
Journal of Chromatography. A
|April 11, 2000
Summary
This study introduces a novel simulated moving-bed (SMB) chromatography method for reversed-phase chromatography, significantly reducing solvent use. The technique optimizes solute distribution coefficients, leading to more concentrated products and lower desorbent consumption.
Area of Science:
- Chemical Engineering
- Chromatography
Background:
- Simulated Moving-Bed (SMB) chromatography offers reduced sorbent and solvent consumption compared to fixed-bed systems.
- Reversed-phase chromatography (RPC) is a common separation technique.
Purpose of the Study:
- To introduce a novel method for further reducing solvent consumption in SMB chromatography for RPC.
- To optimize solute distribution coefficients by varying multi-component mobile phase composition.
Main Methods:
- The method involves adjusting the mobile phase composition to alter solute distribution coefficients.
- The "Triangle-method" by Storti et al. is extended to determine the region of complete separation for this novel SMB approach.
- Theoretical evaluation was performed for the purification of nystatin and separation of insulins.
Main Results:
- Setting desorbent solvent strength higher than feed solvent strength reduces distribution coefficients in the SMB extraction section.
- This leads to easier elution, lower desorbent flow, and potentially more concentrated products.
- Anticipated solvent reduction relative to fixed-bed RP-HPLC was theoretically supported.
Conclusions:
- The proposed novel SMB method effectively reduces solvent consumption in reversed-phase chromatography.
- This technique is versatile and applicable to complex separations like antibiotic purification and protein separation.
- The study provides a theoretical basis for solvent reduction in SMB-RPC.