Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel simulated moving-bed method for reduced solvent consumption.

T B Jensen1, T G Reijns, H A Billiet

  • 1Kluyver Laboratory for Biotechnology, Delft University of Technology, The Netherlands.

Journal of Chromatography. A
|April 11, 2000
PubMed
Summary

This study introduces a novel simulated moving-bed (SMB) chromatography method for reversed-phase chromatography, significantly reducing solvent use. The technique optimizes solute distribution coefficients, leading to more concentrated products and lower desorbent consumption.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autologous growth factor (agf) mixed with allograft increases fixation of cementless implants: an experimental dog study.

Hip international : the journal of clinical and experimental research on hip pathology and therapy·2017
Same author

Impact of metabolic disorders on the relation between overweight/obesity and incident myocardial infarction and ischaemic stroke in fertile women: a nationwide cohort study.

Clinical obesity·2015
Same author

The interrelationships between clinical signs and their effect on involuntary culling among pregnant sows in group-housing systems.

Animal : an international journal of animal bioscience·2012
Same author

Control of the transition temperatures of metallomesogens by specific interface design: application to Mn12 single molecule magnets.

Dalton transactions (Cambridge, England : 2003)·2011
Same author

Association between lean meat percentage and average daily weight gain in Danish slaughter pigs.

Preventive veterinary medicine·2011
Same author

Osteogenic protein-1 increases the fixation of implants grafted with morcellised bone allograft and ProOsteon bone substitute: an experimental study in dogs.

The Journal of bone and joint surgery. British volume·2007

Area of Science:

  • Chemical Engineering
  • Chromatography

Background:

  • Simulated Moving-Bed (SMB) chromatography offers reduced sorbent and solvent consumption compared to fixed-bed systems.
  • Reversed-phase chromatography (RPC) is a common separation technique.

Purpose of the Study:

  • To introduce a novel method for further reducing solvent consumption in SMB chromatography for RPC.
  • To optimize solute distribution coefficients by varying multi-component mobile phase composition.

Main Methods:

  • The method involves adjusting the mobile phase composition to alter solute distribution coefficients.
  • The "Triangle-method" by Storti et al. is extended to determine the region of complete separation for this novel SMB approach.
  • Theoretical evaluation was performed for the purification of nystatin and separation of insulins.

Related Experiment Videos

Main Results:

  • Setting desorbent solvent strength higher than feed solvent strength reduces distribution coefficients in the SMB extraction section.
  • This leads to easier elution, lower desorbent flow, and potentially more concentrated products.
  • Anticipated solvent reduction relative to fixed-bed RP-HPLC was theoretically supported.

Conclusions:

  • The proposed novel SMB method effectively reduces solvent consumption in reversed-phase chromatography.
  • This technique is versatile and applicable to complex separations like antibiotic purification and protein separation.
  • The study provides a theoretical basis for solvent reduction in SMB-RPC.