Related Experiment Video

Updated: Oct 17, 2026

Imaging FITC-dextran as a Reporter for Regulated Exocytosis
04:50

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Published on: June 20, 2018

Histamine release induced by antimicrobial agents and effects of antimicrobial agents on vancomycin-induced histamine

T Toyoguchi1, M Ebihara, F Ojima

  • 1Department of Pharmacy, Yamagata University Hospital, Japan.

The Journal of Pharmacy and Pharmacology
|April 11, 2000
PubMed

Insights

Vancomycin and miconazole trigger histamine release in rat mast cells, but fluconazole does not. Fosfomycin sodium may inhibit vancomycin-induced reactions, suggesting potential therapeutic benefits.

Area of Science:

  • Pharmacology
  • Immunology
  • Toxicology

Background:

  • Anaphylactoid reactions can be associated with vancomycin and certain antifungal agents.
  • Understanding drug-induced histamine release is crucial for patient safety.

Purpose of the Study:

  • To investigate the effects of vancomycin, miconazole, and fluconazole on histamine release from rat peritoneal mast cells.
  • To determine if fosfomycin sodium, cilastatin sodium, or fluconazole can inhibit vancomycin-induced histamine release.

Main Methods:

  • Rat peritoneal mast cells were used to assess histamine release.
  • Dose-dependent effects of vancomycin, miconazole, and fluconazole were evaluated.
  • Inhibition of vancomycin-induced histamine release by other agents was tested at human-equivalent doses.

Main Results:

  • Vancomycin and miconazole induced dose-dependent histamine release.
  • Fluconazole did not provoke histamine release within the tested concentration range.
  • Fosfomycin sodium inhibited vancomycin-induced histamine release, while cilastatin sodium and fluconazole did not.

Conclusions:

  • Vancomycin and miconazole are implicated in histamine release from rat mast cells.
  • Fluconazole appears unlikely to cause histamine release.
  • Fosfomycin sodium demonstrates potential to mitigate vancomycin-induced histamine release.

More Related Videos

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Related Concept Videos

Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists01:28

Acid Suppressive Drugs for Peptic Ulcer Disease: Histamine H2-Receptor Antagonists

Histamine H2 receptors, which are intricately located on the basolateral membrane of parietal cells, play a crucial role in modulating gastric acid secretion. When released from enterochromaffin-like cells, histamine engages H2 receptors, initiating the cyclic AMP (cAMP) pathway. In this pathway, adenylyl cyclase converts ATP into cAMP, elevating intracellular cAMP levels. The activation of protein kinase A follows, stimulating the proton pump. This stimulation prompts the secretion of hydrogen...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
Pathophysiology of Vomiting01:22

Pathophysiology of Vomiting

Vomiting is a complex physiological response to expel harmful or irritating substances from the body. It's a defensive mechanism triggered by stimuli like poisons, microbial toxins, cytotoxic drugs, and mechanical abdominal distension. The process is centrally coordinated by the vomiting (or emetic) center located in the medulla of the brainstem. This area, rich in muscarinic M1, histamine H1, neurokinin 1 (NK1), and serotonin 5-HT3 receptors, coordinates the act of vomiting through interaction...
Allergic Reactions: Anaphylaxis01:30

Allergic Reactions: Anaphylaxis

Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin, heparin),...
Modified-Release Drug Delivery Systems: Stimuli-Activated01:30

Modified-Release Drug Delivery Systems: Stimuli-Activated

Stimuli-activated drug delivery systems are designed to release drugs in response to specific physical, chemical, or biological stimuli. These systems often utilize hydrogels—three-dimensional, hydrophilic polymer networks capable of swelling in aqueous environments and retaining significant fluid volumes. Upon exposure to particular stimuli, these hydrogels undergo structural transitions that allow the embedded drug to be released. Due to this adaptive behavior, such systems are also called...