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Related Experiment Videos

The euglycemic hyperinsulinemic clamp examination: variability and reproducibility.

L Bokemark1, A Frödén, S Attvall

  • 1Department of Medicine, Sahlgrenska University Hospital, Göteborg, Sweden.

Scandinavian Journal of Clinical and Laboratory Investigation
|April 11, 2000
PubMed
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The euglycemic hyperinsulinemic clamp method shows approximately 15% variability. Adjusting the glucose infusion rate for fat-free mass improves reproducibility for assessing insulin sensitivity.

Area of Science:

  • Metabolic research
  • Clinical physiology

Background:

  • The euglycemic hyperinsulinemic clamp is a gold standard for measuring insulin sensitivity.
  • Assessing the reproducibility of this method is crucial for reliable clinical and research applications.

Purpose of the Study:

  • To evaluate the reproducibility of the euglycemic hyperinsulinemic clamp technique.
  • To determine the optimal method for adjusting glucose infusion rate (GIR) for enhanced accuracy.

Main Methods:

  • Recruited 32 healthy 60-year-old men with varying insulin sensitivity.
  • Performed conventional 2-hour clamp examinations twice, 2 weeks apart.
  • Infused insulin and adjusted glucose infusion rate (GIR) based on body weight or fat-free mass (FFM).

Main Results:

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  • The glucose infusion rate during the final hour (GIR60-120) adjusted for FFM demonstrated the highest correlation and lowest coefficient of variation (14.7%).
  • Adjusting GIR for weight, not FFM, underestimated insulin sensitivity in obese men.
  • Measurement error remained constant across all GIR levels.

Conclusions:

  • The euglycemic hyperinsulinemic clamp method exhibits a coefficient of variation of approximately 15%.
  • Adjusting the glucose infusion rate for fat-free mass is recommended for improved reproducibility and accurate assessment of insulin sensitivity.