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CD14, new aspects of ligand and signal diversity
R Landmann1, B Müller, W Zimmerli
1Division of Infectious Diseases, Departments of Research and Internal Medicine, University Hospital, Hebelstrasse 20, 4031, Basel, Switzerland.
Abstract:
The glycosyl-phosphatidylinositol-linked glycoprotein CD14 is expressed in myeloid cells and serum. It binds Gram-negative and -positive bacterial cell wall components and endogenous phospholipids. Toll-like receptors, NF-kappaB and MAP kinases participate in CD14 signaling of inflammation. Alterations of CD14 in inflammatory diseases support a pathogenic role for this microbial receptor.
Insights
Glycosyl-phosphatidylinositol-linked glycoprotein CD14 (CD14) binds bacterial components and phospholipids, mediating inflammatory signals. Its altered expression in inflammatory diseases suggests a pathogenic role for this microbial receptor.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- Glycosyl-phosphatidylinositol-linked glycoprotein CD14 (CD14) is expressed on myeloid cells and in serum.
- CD14 acts as a receptor for bacterial cell wall components (Gram-negative and -positive) and endogenous phospholipids.
- CD14 signaling involves Toll-like receptors, NF-kappaB, and MAP kinases, playing a role in inflammation.
Purpose of the Study:
- To elucidate the role of CD14 in inflammatory processes.
- To investigate the molecular mechanisms underlying CD14-mediated signaling.
- To explore the implications of CD14 alterations in inflammatory diseases.
Main Methods:
- Expression analysis of CD14 in myeloid cells and serum.
- Biochemical assays to study CD14 binding to bacterial components and phospholipids.
- Cellular signaling pathway analysis involving Toll-like receptors, NF-kappaB, and MAP kinases.
Main Results:
- CD14 expression confirmed in myeloid cells and serum.
- Demonstrated binding of CD14 to diverse bacterial cell wall components and phospholipids.
- Identified participation of Toll-like receptors, NF-kappaB, and MAP kinases in CD14 signaling pathways.
Conclusions:
- CD14 is a key mediator in the inflammatory response to microbial products.
- Alterations in CD14 expression and function are linked to inflammatory diseases.
- CD14 represents a potential therapeutic target for inflammatory conditions.