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Related Experiment Videos

Simple microplate method for determination of urinary iodine.

T Ohashi1, M Yamaki, C S Pandav

  • 1Pharmaceutical Research Laboratory, Hitachi Chemical Co., Ltd., 13-1, Higashi-cho 4-chome, Hitachi-shi, Ibaraki-ken 317-8555, Japan. oohashi@hitachi-chem.co.jp

Clinical Chemistry
|April 12, 2000
PubMed
Summary

A new microplate method accurately measures urinary iodine, crucial for monitoring iodine deficiency. This rapid technique simplifies analysis for public health initiatives.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Public Health

Background:

  • Urinary iodine concentration is a key indicator for assessing iodine deficiency disorders.
  • Existing methods for urinary iodine analysis can be time-consuming and complex.
  • Development of a simplified, rapid, and quantitative assay is needed for effective public health monitoring.

Purpose of the Study:

  • To develop and validate a novel microplate-based method for the quantitative determination of urinary iodine.
  • To adapt both the digestion and Sandell-Kolthoff reaction steps into a microplate format for improved efficiency.

Main Methods:

  • Ammonium persulfate digestion was performed in a microplate under sealed conditions at 110°C for 60 minutes.
  • The Sandell-Kolthoff reaction was conducted in a transparent microplate at 25°C for 30 minutes.

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  • Urinary iodine levels were measured using a microplate reader at 405 nm.

    • Main Results:

    • The method demonstrated high accuracy with a mean iodine recovery of 98% and a low theoretical detection limit of 0.11 µmol/L.
    • Excellent precision was observed, with intra- and inter-assay coefficients of variation below 10% for relevant iodine concentrations.
    • The developed microplate method showed strong agreement with conventional chloric acid digestion and inductively coupled plasma mass spectrometry methods.

    Conclusions:

    • The integrated microplate method offers a simple, rapid, and quantitative approach for urinary iodine measurement.
    • This assay is well-suited for the routine monitoring of iodine status and the control of iodine deficiency disorders.