Promoter elements involved in the expression of the Aspergillus parasiticus aflatoxin biosynthesis pathway gene avnA
J W Cary1, B G Montalbano, K C Ehrlich
1Southern Regional Research Center, United States Department of Agriculture, PO Box 19687, 1100 R.E. Lee Blvd., New Orleans, LA, USA. jcary@nola.srrc.usda.gov
Abstract:
One of the early genes in aflatoxin biosynthesis, avnA, encodes a pathway-specific cytochrome P-450 monooxygenase that catalyzes the hydroxylation of the polyketide anthraquinone, averantin. Based on beta-glucuronidase (GUS) reporter and electrophoretic mobility shift assays, promoter sites upstream of -118 bp in the 367-bp verB-avnA intergenic region are not required for avnA gene activity. Therefore, only the -100 to -110 site of the four putative binding sites for AFLR, the aflatoxin biosynthetic pathway transcription regulatory protein (consensus binding sequence: 5'-TCGN(5)CGR-3') was required for elevated avnA expression.
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