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Related Experiment Videos

The human RGL (RalGDS-like) gene: cloning, expression analysis and genomic organization.

R Sood1, I Makalowska, J D Carpten

  • 1Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Building 36, Room 3D05, 9000 Rockville Pike, Bethesda, MD, USA. rsood@nhgri.nih.gov

Biochimica Et Biophysica Acta
|April 13, 2000
PubMed
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This summary is machine-generated.

Two forms of the human RGL gene, differing at the amino terminus, were cloned and localized. These RGL gene variants are involved in Ras and Ral signaling pathways.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Signaling

Background:

  • Ral GDP dissociation stimulator (RalGDS) family proteins, including RGL, RLF, and RGL2, are key downstream effectors in Ras and Ral signaling pathways.
  • Understanding the precise structure and expression of these proteins is crucial for elucidating their roles in cellular processes.

Purpose of the Study:

  • To precisely localize and clone two distinct forms of the human RGL gene.
  • To characterize the structural differences between these human RGL gene transcripts.
  • To investigate the tissue-specific expression patterns of human RGL.

Main Methods:

  • Cloning of human RGL gene transcripts from liver and brain cDNA libraries.
  • Genomic analysis to identify alternative exon usage.

Related Experiment Videos

  • Northern blot analysis for tissue expression profiling.

    • Main Results:

    • Two human RGL gene transcripts (Transcript A and Transcript B) were identified, differing in their amino-terminal coding sequences.
    • Transcript A shares the amino terminus with mouse RGL, while Transcript B exhibits a 45-amino acid substitution.
    • Genomic analysis revealed alternative splicing at exon 1, with Transcript B utilizing two alternative exons (1B1 and 1B2).
    • The human RGL protein shows high amino acid identity (94%) to its mouse counterpart.
    • Northern blot analysis demonstrated widespread expression of human RGL across various tissues, with notable high expression in the heart, brain, kidney, spleen, and testis.

    Conclusions:

    • The human RGL gene exists in at least two transcript forms due to alternative splicing, impacting the amino terminus.
    • These findings provide a detailed molecular understanding of RGL gene structure and its differential expression in human tissues.
    • The characterized RGL variants are likely to play significant roles in the Ras and Ral signaling pathways across multiple organs.