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Cell wall components of Staphylococcus aureus induce interleukin-5 production in patients with atopic dermatitis
K Matsui1, R Motohashi, A Nishikawa
1Department of Immunobiology, Meiji Pharmaceutical University, Tokyo, Japan. kmatsui@my-pharm.ac.jp
Abstract:
Lipoteichoic acid (LTA) and peptidoglycan (PEG) from Staphylococcus aureus induced interleukin-5 (IL-5) production in human peripheral blood mononuclear cells (PBMC) from patients with atopic dermatitis (AD) but not in PBMC from healthy donors. The production of IL-5 induced by LTA or PEG was correlated with the expression of IL-5 mRNA in PBMC. Furthermore, the level of IL-5 production induced by treatment with both LTA and PEG from S. aureus was higher than that induced by the addition of each alone. These results suggest that LTA and PEG have an additive effect on IL-5 production in PBMC from AD patients and may explain the role of colonization with nontoxin-producing strains of S. aureus in these patients.
Insights
Staphylococcus aureus components, lipoteichoic acid (LTA) and peptidoglycan (PEG), stimulate interleukin-5 (IL-5) production in atopic dermatitis (AD) patients. This suggests a role for S. aureus colonization in AD pathogenesis.
Area of Science:
- Immunology
- Microbiology
- Dermatology
Background:
- Atopic dermatitis (AD) is a chronic inflammatory skin condition.
- Staphylococcus aureus colonization is common in AD patients.
- The role of specific bacterial components in AD pathogenesis requires further elucidation.
Purpose of the Study:
- To investigate the effect of Staphylococcus aureus lipoteichoic acid (LTA) and peptidoglycan (PEG) on interleukin-5 (IL-5) production.
- To compare the response in peripheral blood mononuclear cells (PBMC) from AD patients and healthy donors.
Main Methods:
- Human peripheral blood mononuclear cells (PBMC) were isolated from AD patients and healthy controls.
- PBMC were stimulated with LTA and PEG from S. aureus.
- Interleukin-5 (IL-5) production and IL-5 mRNA expression were measured.
Main Results:
- LTA and PEG induced significant IL-5 production in PBMC from AD patients, but not from healthy donors.
- IL-5 production correlated with IL-5 mRNA expression.
- Combined LTA and PEG treatment showed an additive effect on IL-5 production.
Conclusions:
- LTA and PEG from S. aureus can induce IL-5 production in AD patients' PBMC.
- These findings suggest a potential mechanism by which S. aureus colonization contributes to AD.
- The additive effect of LTA and PEG highlights their combined role in immune response modulation in AD.