Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

7alpha-Hydroperoxycholesterol causes CNS neuronal cell death.

H Kölsch1, M Ludwig, D Lütjohann

  • 1Department of Clinical Biochemistry, University of Bonn, Germany.

Neurochemistry International
|April 13, 2000
PubMed
Summary

Oxysterols, like 7alpha-hydroperoxycholesterol, cause neuronal cell death through necrosis. This study shows its cytotoxic effects on human neuroblastoma cells, highlighting potential neurotoxicity beyond its role in atherosclerosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma oxyphytosterols most likely originate from hepatic oxidation and subsequent spill-over in the circulation.

The Journal of steroid biochemistry and molecular biology·2021
Same author

Interaction of chylomicron remnants and VLDLs during ultracentrifuge separation based on the Svedberg flotation rate.

Journal of internal medicine·2019
Same author

[Sitosterolemia (phytosterolemia)].

Der Internist·2019
Same author

The isoflavones genistein and daidzein increase hepatic concentration of thyroid hormones and affect cholesterol metabolism in middle-aged male rats.

The Journal of steroid biochemistry and molecular biology·2019
Same author

Plant-based sterols and stanols in health & disease: "Consequences of human development in a plant-based environment?"

Progress in lipid research·2019
Same author

Modulation of cholesterol-related sterols during Eimeria bovis macromeront formation and impact of selected oxysterols on parasite development.

Molecular and biochemical parasitology·2018

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Brain cholesterol is crucial for neuronal membranes and can be oxidized into cytotoxic oxysterols.
  • Oxysterols are implicated in various pathologies, including neurodegenerative diseases and atherosclerosis.

Purpose of the Study:

  • To investigate the neurotoxic effects of 7alpha-hydroperoxycholesterol on human neuroblastoma cells (SH-SY5Y).
  • To elucidate the mechanism of cell death induced by this specific oxysterol.

Main Methods:

  • Utilized the human neuroblastoma cell line SH-SY5Y.
  • Assessed cell viability using cell morphology and Trypan blue exclusion.
  • Measured DNA fragmentation, caspase-3 activity, lactate dehydrogenase (LDH) release, and reactive oxygen species (ROS) generation.

Related Experiment Videos

Main Results:

  • 7alpha-hydroperoxycholesterol induced dose-dependent neuronal cell death.
  • At 48 hours, 10 microM resulted in 50% cell death, while 50 microM caused 92% cell death.
  • Rapid LDH release and ROS generation were observed, but DNA fragmentation and caspase-3 activity were not detected.

Conclusions:

  • 7alpha-hydroperoxycholesterol induces necrosis in neuronal cells.
  • The mechanism involves rapid LDH release and ROS generation, independent of apoptosis.
  • These findings suggest a direct neurotoxic role for 7alpha-hydroperoxycholesterol.