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Ultracytochemical localization of 5'-nucleotidase activity in human ejaculated spermatozoa
T Takayama1, S Matsubara, H Shibahara
1Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan. obtakegy@jichi.ac.jp
International Journal of Andrology
|April 13, 2000
Abstract:
5'-nucleotidase, an adenosine producing enzyme with a glycosylphosphatidylinositol-anchored structure, was localized in human ejaculated spermatozoa. The poly-L-lysine-coated dish method was used to prepare the specimens, and the cerium method was employed for electron-microscopic enzyme localization. Precipitates indicating enzyme activity were detected on the outer side of the external plasma membrane of the acrosomal region. This enzyme may play a role in sperm motility and male infertility.