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Updated: Oct 17, 2026

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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Published on: January 7, 2019

Differential expression of chemokine receptor CXCR4 in Th1 and Th2 subtypes of CD4+ lymphocytes

P P Jagodziński1, W H Trzeciak

  • 1Department of Physiological Chemistry, University of Medical Sciences, Poznań, Poland. pjagodzi@eucalyptus.usoms.poznan.pl

Folia Histochemica Et Cytobiologica
|April 14, 2000
PubMed

Different cytokine profiles allow to divide the CD4+ lymphocytes into Th1, Th2 and Th0 subtypes. It has been observed that the Th2 cells are more efficient supporters for HIV-1 replication than the Th1 cells. The Th1 and the Th2 cells were isolated from peripheral blood lymphocytes of HIV-1 seronegative individuals and the density of CXCR4 receptors was determined by flow cytometry using antibodies directed against the CXCR4 receptor. Flow cytometric analysis revealed higher expression of the HIV-1 co-receptor CXCR4 on Th2 cells than on the Th1, which might explain better replication of HIV-1 viruses in the Th2 cells.

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