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Illegitimate villin transcripts in normal bone marrow precludes detection of colon cancer micrometastases
C Salbe1, P de Cremoux, C Bonneton
1Center for the Study of Biological Markers of Malignancy, Venice Regional Hospital, Italy.
The International Journal of Biological Markers
|April 14, 2000
Abstract:
Villin is a specific marker for normal and tumoral colon tissue. We have developed a highly sensitive assay using reverse transcription (RT) and real-time PCR to detect villin transcripts. The sensitivity of detection is one colon cancer cell. However, high levels of illegitimate villin transcripts were observed in normal bone marrow, precluding the use of villin RT-PCR for routine detection of colon cancer cells in bone marrow of patients with colon cancer.