Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evolutionary conservation in protein folding kinetics.

K W Plaxco1, S Larson, I Ruczinski

  • 1Department of Biochemistry, University of Washington, Seattle, WA 98195, USA. kwplaxco@u.washington.edu

Journal of Molecular Biology
|April 15, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pyelo-Nephritis Consequent upon the Presence of Calculi and Over-distention of the Bladder for Three Weeks-Twenty-one Calculi Found in the Bladder.

Buffalo medical and surgical journal·2023
Same author

Arsenical Poisoning-The "Kloss Case"-Unusual Post-mortem Appearances.

Buffalo medical and surgical journal·2023
Same author

"Sewer Gas and Its Dangers," Letter from Prof. F. H. Hamilton.

Buffalo medical and surgical journal·2023
Same author

"Sewer Gas" and Its Dangers.

Buffalo medical and surgical journal·2023
Same author

Diseases of the Scalp.-The Syphilides: A Clinical Lecture Delivered at the Hospital of the Sisters of Charity.

Buffalo medical and surgical journal·2023
Same author

Hæmaturia as a Symptom of Diseases of the Genito-Urinary Organs.

Buffalo medical and surgical journal·2023
Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026
Same journal

Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

Journal of molecular biology·2026
Same journal

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

Journal of molecular biology·2026
Same journal

UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

Journal of molecular biology·2026
Same journal

Structural basis for the pro-amyloidogenic action and ligand binding of a novel W72R variant of human apolipoprotein A-I.

Journal of molecular biology·2026
Same journal

Cryo-EM Structure of the C. elegans Septin Tetramer Reveals a Revised Architecture and Conserved Positional Orthology.

Journal of molecular biology·2026
See all related articles

Protein folding transition states show conserved structures but not specific stabilizing interactions. Evolutionary conservation of residues does not always correlate with their role in transition state structure.

Area of Science:

  • Protein folding dynamics
  • Molecular evolution
  • Biophysics

Background:

  • Theoretical predictions suggest sequence and structural conservation of protein folding transition states.
  • Experimental validation is needed to confirm these predictions.

Purpose of the Study:

  • To experimentally test the theoretical predictions regarding the conservation of protein folding transition states.
  • To investigate the correlation between residue conservation and their roles in transition state structure.

Main Methods:

  • Utilized homologous sequence alignments of proteins.
  • Employed coupled mutagenesis and kinetics studies for protein characterization.
  • Analyzed correlations between residue roles in transition state structure and evolutionary conservation.

Related Experiment Videos

Main Results:

  • Only one out of six characterized proteins showed a significant correlation between residue roles in transition state structure and evolutionary conservation.
  • A significant correlation was found between the contributions of individual sequence positions to transition state structure across homologous proteins.
  • The overall structure of the folding transition state ensemble appears more conserved than specific stabilizing interactions.

Conclusions:

  • The folding transition state ensemble's structure is more conserved than the specific interactions that stabilize it.
  • Evolutionary conservation is not a perfect predictor of a residue's role in stabilizing the transition state structure.