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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
10:00

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

Regulation of SOCS-1 expression by translational repression

A Gregorieff1, S Pyronnet, N Sonenberg

  • 1McGill Cancer Centre and the Departments of Biochemistry, Oncology, and Medicine, McGill University, Montréal, Québec H3G 1Y6, Canada.

The Journal of Biological Chemistry
|April 15, 2000
PubMed

Insights

Suppressor of cytokine signaling (SOCS) expression is regulated by multiple mechanisms. This study reveals a novel translational repression of SOCS-1, mediated by its 5' untranslated region, adding another layer to cytokine signaling control.

Area of Science:

  • Molecular Biology
  • Immunology
  • Cell Signaling

Background:

  • Cytokine receptor signaling is crucial for cellular communication and immune responses.
  • Suppressor of cytokine signaling (SOCS) proteins are key negative regulators of cytokine signaling.
  • Existing regulatory mechanisms for SOCS expression include transcriptional control and protein stability via elongins B and C.

Purpose of the Study:

  • To investigate novel regulatory mechanisms controlling SOCS protein expression.
  • To elucidate the role of translational control in SOCS-1 regulation.
  • To identify the specific elements within SOCS-1 responsible for translational repression.

Main Methods:

  • Analysis of SOCS-1 gene expression and protein levels.
  • Structure-function analysis of the 5' untranslated region (UTR) of socs-1.
  • Investigation of cap-dependent translation and modulation by eIF4E-binding proteins.

Main Results:

  • SOCS-1 expression is significantly repressed at the level of translation initiation.
  • The 5' UTR of socs-1, containing two upstream AUGs, mediates this translational repression.
  • SOCS-1 translation is cap-dependent and influenced by eIF4E-binding proteins.

Conclusions:

  • A novel mechanism of translational repression regulates SOCS-1 expression.
  • Multiple regulatory layers, including translation, ensure precise control of SOCS expression.
  • This intricate regulation prevents inappropriate interference with cytokine-mediated effects and maintains immune homeostasis.

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