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Extracorporeal plateletpheresis induces the interaction of activated platelets with white blood cells
K Gutensohn1, A Alisch, W Krueger
1Transfusion Medicine/Transplantation Immunology, University Hospital Eppendorf, University of Hamburg, Hamburg, Germany. gutensohn@uke.uni-hamburg.de
Background And Objectives:
In this study we investigated whether platelet activation during apheresis results in the binding of platelets to white blood cells.
Material And Methods:
Analysis of platelet-leukocyte interaction was performed using multiparameter, three-color flow cytometry.
Results:
Over the duration of the procedure, there was an increase in the surface expression of CD62p (P-selectin) and CD63 (p<0.05), and also in the binding of platelets to monocytes (p<0.05), neutrophilic granulocytes (p<0.05) and to CD3+ cells (initially to a low degree; p<0.05). Platelet binding to CD19+ cells did not change significantly.
Conclusion:
This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.
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