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Related Experiment Video

Updated: Jul 12, 2026

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

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[Synthesis and immunochemical properties of the recombinant major surface glycoprotein E2 of the classical swine

A V Krivonos, A D Zaberezhny, T V Grebennikova

Voprosy Virusologii

|April 15, 2000

Summary

Recombinant E2 protein from classical swine fever virus (CSFV) was successfully synthesized in insect cells. This purified protein is effective for detecting CSFV antibodies using competitive enzyme immunoassay.

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Engineering Antiviral Agents via Surface Plasmon Resonance

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Published on: June 14, 2022

Area of Science:

Molecular biology
Virology
Biotechnology

Context:

Classical swine fever virus (CSFV) poses a significant threat to swine health and the global pork industry.
Effective diagnostic tools are crucial for controlling CSFV outbreaks.
Recombinant protein expression offers a scalable and safe alternative for antigen production.

Purpose:

To develop a method for producing recombinant E2 protein of CSFV in insect cell culture.
To optimize expression conditions for maximum yield and accumulation of the E2 protein.
To purify and validate the recombinant E2 protein for use in diagnostic assays.

Summary:

Recombinant E2 protein from both vaccine and virulent CSFV strains was synthesized in SF-21 and High-Five cells using a baculovirus expression system.
Optimization involved removing the transmembrane domain and adding a signal polypeptide for secretion, with peak expression observed at 48-96 hours post-infection.
The expressed E2 protein, at levels of 5-10 µg/10^6 cells, was purified and confirmed for specificity, making it suitable for detecting CSFV antibodies.

Impact:

Provides a reliable and specific recombinant antigen for developing sensitive diagnostic tests for classical swine fever.
Facilitates improved disease surveillance and control strategies for CSFV.
Contributes to the advancement of biotechnological approaches in veterinary diagnostics.