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Related Experiment Videos

Embryonic and genetic manipulation in fish.

Z Y Zhu1, Y H Sun

  • 1State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Luojiashan, Wuhan. zyzhu@ihb.ac.cn

Cell Research
|April 15, 2000
PubMed
Summary

Fish nuclear transplantation and gene transfer techniques have enabled the creation of cloned and transgenic fish. These advancements, including somatic cell cloning and growth hormone gene modification, offer potential for developing valuable fish strains.

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Area of Science:

  • * Aquaculture and Developmental Biology
  • * Genetics and Molecular Biology

Background:

  • * Fishes are diverse vertebrate models for cell and developmental biology research.
  • * Nuclear transplantation in fish, initiated in the 1960s, involves transferring embryonic nuclei into enucleated eggs.
  • * Somatic cell nuclear transfer in goldfish demonstrated the first cloned fish from somatic cells.

Purpose of the Study:

  • * To explore the potential of nuclear transplantation and gene transfer in fish.
  • * To develop methods for creating cloned and transgenic fish with enhanced traits.
  • * To investigate the behavior and integration of foreign genes in fish embryogenesis.

Main Methods:

  • * Nuclear transplantation using embryonic and cultured somatic cell nuclei into enucleated fish eggs.

Related Experiment Videos

  • * Microinjection of recombinant MThGH gene into fish eggs for transgenic fish production.
  • * Development of an "all-fish" gene construct (CAgcGH) for enhanced growth.

    • Main Results:

    • * Successful generation of nucleo-cytoplasmic hybrids and fertile cloned fish from somatic cells.
    • * Production of the first transgenic fish (1984) carrying the MThGH gene, showing enhanced growth and protein utilization.
    • * Transgenic common carp exhibited 2-4 times increased growth rate and doubled body weight.
    • * Yellow River Carp with the CAgcGH construct showed significantly fast-growth traits.

    Conclusions:

    • * Nuclear transplantation and gene transfer are effective for producing cloned and transgenic fish.
    • * Somatic cell reprogramming and gene modification can lead to enhanced fish growth and efficiency.
    • * Combining cell culture, gene transformation, and nuclear transplantation can generate homogeneous, valuable transgenic fish strains.