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Updated: Oct 16, 2026

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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
Published on: May 28, 2019
A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae
1Department of Molecular Genetics, Max-Planck-Institute for Biophysical Chemistry, D-37070, Göttingen, Germany. aanna@gwdg.de
Gene
|April 18, 2000
Abstract:
Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly.

