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Published on: February 24, 2026
Automated surgical equipment requires routine disinfection of vacuum control manifold to prevent postoperative
H Miño de Kaspar1, T Grasbon, A Kampik
1Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany.
Purpose:
Contamination of automated surgical equipment is a potential source of postoperative endophthalmitis. The effect of disinfecting the unsterile vacuum control manifold (VCM) on contamination of the aspiration fluid was studied.
Design:
Comparative prospective microbiologic contamination study. SPECIMENS AND CONTROLS: The 37 aspiration fluid specimens studied or examined consisted of 25 from three automated evacuation systems equipped with an internal VCM (experimental groups) and 12 from one system equipped with a modified external VCM (control group). In addition, the tubings of two internal VCMs were investigated.
Methods:
We investigated aspiration fluid specimens from routine cataract and vitrectomy operations performed with automated evacuation systems. After tracing the source of contamination to the internal VCM, the system was modified, creating an external VCM with the possibility for disinfection, and the study was continued. Whereas no sterilizing rinsing procedures were applied to the hidden internal VCM, the modified external VCM was regularly rinsed and filled overnight with 70% isopropanol. All samples were collected under sterile conditions, centrifuged, and cultured for bacterial growth on blood agar and MacConkey agar for 24 to 48 hours at 37 degrees C. The samples of the two internal VCMs were cultured for fungi as well.
Main Outcome Measures:
Bacterial growth was quantified, and bacteria and fungi were isolated and identified according to standard microbiologic procedures.
Results:
In all aspiration fluid specimens from internal VCM-systems, 2+ to 4+ bacterial growth was found. Pseudomonas-related genera (Stenotrophomonas maltophilia, 17x; Comamonas acidovorans, 8x; Chryseomonas spp., 3x), Agrobacterium radiobacter (1 3x), Flavobacterium spp. (3x), and Micrococcus luteus (7x) were found most frequently. The tubing of the two VCMs contained biofilms exhibiting several of these bacteria and fungi. All specimens from the modified external VCM-system remained sterile. There was a significant difference with regard to the frequency of contamination of the aspiration fluid between the experimental and control groups (chi-square: P = 0.0001).
Conclusions:
The technical modification described herein allows facile hygienic measures, by which contamination of aspiration fluid by the VCM can be eliminated, which otherwise can be a significant source of contamination.
Insights
Disinfecting the vacuum control manifold (VCM) in surgical equipment prevents contamination of aspiration fluid. Modifying the VCM to an external, disinfected unit eliminated bacterial and fungal growth, significantly reducing infection risk.
Area of Science:
- Ophthalmology
- Microbiology
- Surgical Technology
Background:
- Contamination of automated surgical equipment poses a risk for postoperative endophthalmitis.
- The vacuum control manifold (VCM) in automated surgical systems can harbor microorganisms.
Purpose of the Study:
- To evaluate the impact of disinfecting the unsterile vacuum control manifold (VCM) on aspiration fluid contamination.
- To assess the efficacy of a modified external VCM design in preventing microbial contamination.
Main Methods:
- A comparative prospective microbiologic study was conducted on 37 aspiration fluid specimens.
- Specimens were collected from automated evacuation systems with internal VCMs (experimental) and a modified external VCM (control).
- Cultures were performed for bacterial and fungal growth, with identification of isolates.
Main Results:
- All internal VCM specimens showed significant bacterial growth (2+ to 4+), with Pseudomonas-related genera being most frequent.
- Biofilms containing bacteria and fungi were identified in the tubing of internal VCMs.
- Specimens from the modified external VCM remained sterile, showing a significant reduction in contamination (chi-square: P = 0.0001).
Conclusions:
- Modifying the VCM to an external, disinfected unit effectively eliminates aspiration fluid contamination.
- This technical modification facilitates hygienic measures, mitigating the risk of endophthalmitis from VCM contamination.
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