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Updated: Jul 15, 2026

DNA Methylation: Bisulphite Modification and Analysis
Published on: October 21, 2011
Quantitative DNA methylation analysis by fluorescent polymerase chain reaction single-strand conformation
1First Department of Internal Medicine, Sapporo Medical University, Japan.
Abstract:
A novel DNA methylation assay technique, termed bisulfite single-strand conformation polymorphism (bisulfite-SSCP), is a combination of sodium-bisulfite modification and fluorescence-based polymerase chain reaction (PCR)-SSCP. After bisulfite treatment followed by PCR amplification, methylated and unmethylated alleles can be simultaneously separated in a nondenaturing gel using an automated DNA sequencer. Using bisulfite-SSCP, methylation of hMLH1 was detected in a quantitative manner. This method is not only simple, quick, accurate, and quantitative, but detailed information about methylation is also available with less work. Methylation analysis of large numbers of samples for multiple loci will be facilitated by bisulfite-SSCP.
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