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Related Experiment Videos

Humanized in vivo model for streptococcal impetigo.

D A Scaramuzzino1, J M McNiff, D E Bessen

  • 1Departments of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut.

Infection and Immunity
|April 18, 2000
PubMed
Summary

Researchers developed a new in vivo model for group A streptococcal (GAS) impetigo using human skin on mice. This model effectively replicates GAS skin infections, showing bacterial growth and distinct pathological features.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Dermatology

Background:

  • Group A Streptococcus (GAS) is a significant human pathogen.
  • GAS causes various infections, including impetigo and pharyngitis.
  • Developing accurate in vivo models is crucial for studying GAS pathogenesis.

Purpose of the Study:

  • To establish a novel in vivo model for studying group A streptococcal (GAS) impetigo.
  • To investigate the pathological progression of GAS skin infections in a humanized mouse model.
  • To compare the virulence of different GAS emm patterns in skin infections.

Main Methods:

  • Human neonatal foreskin grafted onto SCID mice was used as the model system.
  • Superficial skin damage was induced, followed by topical application of virulent GAS.

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  • Infection progression, bacterial load (CFU), and host inflammatory response were monitored over 7 days.

    • Main Results:

    • A severe impetigo-like infection was successfully induced with low CFU of GAS.
    • Histological analysis revealed stratum corneum loss, bacterial adherence, and neutrophil infiltration.
    • GAS demonstrated significant reproduction in vivo, with a 20- to 14,000-fold increase in CFU.
    • emm pattern D strains exhibited higher pathology scores compared to emm pattern A, B, or C strains.

    Conclusions:

    • The developed model accurately mimics human GAS impetigo and ecthyma.
    • The model allows for the study of GAS pathogenesis and the evaluation of different GAS strains.
    • Findings support the epidemiological association between emm pattern D strains and impetigo prevalence.