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Biochemical and genetic evidence for phospholipase C activity in Mycobacterium ulcerans
A Gomez1, A Mve-Obiang, B Vray
1Mycobacteriology Unit, Institute of Tropical Medicine, B-2000 Antwerp, Belgium. arley@itg.be
Infection and Immunity
|April 18, 2000
Abstract:
This study reports the existence of phospholipase C and D enzymatic activities in Mycobacterium ulcerans cultures as determined by use of thin-layer chromatography to detect diglycerides in hydrolysates of radiolabeled phosphatidylcholine. M. ulcerans DNA sequences homologous to the genes encoding phospholipase C in Mycobacterium tuberculosis and Pseudomonas aeruginosa were identified by sequence analysis and DNA-DNA hybridization. Whether or not the phospholipase C and D enzymes of M. ulcerans plays a role in the pathogenesis of the disease needs further investigation.