General method for HPLC purification and sequencing of selected dsDNA gene fragments from complex PCRs generated during gene expression profiling
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DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
Published on: January 27, 2016
L Y Wong1, V Belonogoff, V L Boyd
1Celera Genomics, Foster City, CA, USA.
Biotechniques
|April 19, 2000
Abstract:
Gene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50-500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represent differentially expressed genes. The gene fragments are sequenced directly after vacuum drying of the collected HPLC fractions.
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