Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A negative autoregulatory link between Nramp1 function and expression.

S T Baker1, C H Barton, T E Biggs

  • 1University of Southampton, Biochemistry and Molecular Biology, United Kingdom.

Journal of Leukocyte Biology
|April 19, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DOSIMETRIC QUALITY ASSURANCE INTERPRETED FOR ISO 17025 IN PUBLIC HEALTH ENGLAND'S PERSONAL DOSIMETRY SERVICE.

Radiation protection dosimetry·2016
Same author

USE OF A SIMPLE THERMALISED NEUTRON FIELD FOR QUALITY ACCEPTANCE OF WHOLE BODY TLDS.

Radiation protection dosimetry·2016
Same author

Monte Carlo modelling of 90Sr/90Y and 85Kr beta fields for Hp(3) measurements.

Radiation protection dosimetry·2013
Same author

Type testing of a head band dosemeter for measuring eye lens dose in terms of H(P)(3).

Radiation protection dosimetry·2013
Same author

Estimating dual-energy X-ray absorptiometry-derived total body skeletal muscle mass using single-slice abdominal magnetic resonance imaging in obese subjects with and without diabetes: a pilot study.

European journal of clinical nutrition·2012
Same author

Long-term stability of Harshaw LiF:Mg,Cu,P TLDS.

Radiation protection dosimetry·2011
Same journal

Correction to: IL-27 signaling negatively regulates FcɛRI-mediated mast cell activation and allergic response.

Journal of leukocyte biology·2026
Same journal

Autofluorescence enables detection and quantification of EETosis in human leukocyte cultures.

Journal of leukocyte biology·2026
Same journal

Cold Atmospheric Plasma as an Immunomodulator: Suppression of T-cell Hyperactivation and Graft-versus-Host Disease via Redox Regulation.

Journal of leukocyte biology·2026
Same journal

Distinct lymphocyte immune signatures to nivolumab and recombinant IL-7 ex vivo in patients with sepsis.

Journal of leukocyte biology·2026
Same journal

Eosinophil Essentials: Identification and Assessment of Activity.

Journal of leukocyte biology·2026
Same journal

Immune cell landscape reveals 5 immune-related subtypes and molecular characteristics with prognostic and therapeutic implications in pan-cancer.

Journal of leukocyte biology·2026
See all related articles

Natural resistance-associated macrophage protein 1 (Nramp1) enhances resistance to intracellular pathogens by regulating macrophage iron. Inflammatory stimuli, iron, and sodium nitroprusside up-regulate Nramp1, impacting macrophage iron homeostasis.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Natural resistance-associated macrophage protein 1 (Nramp1) is crucial for host defense against intracellular pathogens.
  • Nramp1 modulates the macrophage environment by altering iron availability, influencing pathogen survival.
  • The regulation of Nramp1 expression and its downstream effects on macrophage function require further elucidation.

Purpose of the Study:

  • To investigate the impact of inflammatory stimuli, iron, and sodium nitroprusside on Nramp1 expression in bone marrow-derived macrophages.
  • To determine the functional consequences of Nramp1 expression on macrophage growth and iron metabolism.
  • To explore the potential for Nramp1 autoregulation in maintaining macrophage iron homeostasis.

Main Methods:

Related Experiment Videos

  • Bone marrow-derived macrophages were treated with inflammatory stimuli, iron, and sodium nitroprusside.
  • Nramp1 expression was assessed via Western blotting (amino-terminal antibody) and mRNA analysis.
  • Macrophage cell lines expressing Nramp1 were analyzed for growth rates, iron availability (IRP2 activity), and protein kinase Cbeta-1 expression.

    • Main Results:

    • Inflammatory stimuli, iron, and sodium nitroprusside all significantly up-regulated Nramp1 expression, correlating with increased Nramp1 mRNA and protein levels.
    • Macrophage cell lines engineered to express Nramp1 exhibited reduced growth rates.
    • Nramp1 expression led to decreased iron availability, evidenced by increased IRP2 activity and reduced protein kinase Cbeta-1 levels.

    Conclusions:

    • Nramp1 expression is positively regulated by inflammatory signals and iron availability.
    • Nramp1 activity impairs macrophage growth, likely through iron sequestration.
    • A negative autoregulatory loop involving Nramp1 may be critical for maintaining iron homeostasis and low cytoplasmic redox-active iron in macrophages.