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Updated: Jul 30, 2026

Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
Published on: December 20, 2011
Chick muscle-derived protein 62: a novel neurite outgrowth promoting protein
A Uyeda1, M Simo Wydisdtuti, H Nishimune
1Division of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan.
Abstract:
A 3.2 kb chick cDNA clone that coded for a novel muscle-derived protein, MDP62, was isolated from a cDNA library of the denervated crus muscles using an antibody which inhibited the neurite (dendritic and axonal processes) outgrowth activity. MDP62 consisted of 539 aa with a calculated molecular mass of 62 k. The predicted protein sequence was hydrophilic and exhibited an extended coiled-coil domain and a leucine zipper motif. A recombinant protein promoted the neurite outgrowth from the cultured chick neurons of the telencephalon in a dose dependent manner. Northern blotting revealed that MDP77 was ubiquitously expressed. In the transfected COS-7 cells with the cDNA of the epitope-tagged MDP62, the expressed protein was detected in the culture medium, suggesting that the MDP62 might be secreted.
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