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An Improved and High Throughput Respiratory Syncytial Virus (RSV) Micro-neutralization Assay
Published on: January 26, 2019
Subgroup determination of respiratory syncytial virus by RT-PCR
S Tantivanich1, K Suphanaranonda, K Balachanda
1Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Abstract:
PCR optimization for differentiation of RSV subgroup A (RT-PCR-1) and RSV subgroup B (RT-PCR-2) were developed. Various conditions of RT-PCR-1 and RT-PCR-2 were summarized. These methods are highly specific and sensitive to differentiate RSV subgroup A and RSV subgroup B from the other respiratory viruses.
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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