HLA class I antibodies in thalassemic patients

This study was undertaken to demonstrate the prevalence of HLA class I antibodies among 62 polytransfused patients. The diagnosis included beta-thalassemia major, beta-thalassemia/Hb E disease and severe Hb H disease. Their ages ranged from 1 year to 23 years with the mean age of 10.7 years. The number of packed red cell transfusions ranged from 3 to 235 with the mean of 60 episodes per patient. The standard microlymphocytotoxicity test was performed using 50 panels of lymphocytes which specifically identified the majority of HLA class I antibodies. 31/62 cases (50%) were positive for HLA class I antibodies. The detection of single or multiple antibodies depended upon the number of blood transfusions and the patients' ages. These antibodies were induced by the leukocytes present in the transfused packed red cells. Therefore, leukocyte-reduced packed red cells prepared by either additional inverted centrifrugation or leukocyte filter is suggested for the routine blood bank service.