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Related Experiment Videos

Emx1-specific expression of foreign genes using "knock-in" approach.

X L Jin1, H Guo, C Mao

  • 1Department of Molecular and Integrative Physiology, Neuroscience Program, Urbana, Illinois 61801, USA.

Biochemical and Biophysical Research Communications
|April 25, 2000
PubMed
Summary

Researchers created Emx1-specific transgenic mice using ES cell technology. This allows targeted gene expression in the cerebral cortex and hippocampus for developmental studies.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Emx1 gene is homologous to Drosophila empty spiracles and specifically expressed in the cerebral cortex and hippocampus.
  • A restricted expression pattern makes the Emx1 promoter desirable for targeted gene expression in transgenic models.
  • Understanding Emx1's role is crucial for studying brain development and plasticity.

Purpose of the Study:

  • To characterize the Emx1 promoter for directing foreign gene expression in transgenic mice.
  • To create a transgenic mouse line with Emx1-specific expression of reporter and cre genes.
  • To validate the utility of this model for future research on cortical and hippocampal development.

Main Methods:

  • Utilized embryonic stem (ES) cell technology to insert lacZ reporter and cre genes into exon 1 of the Emx1 gene.

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  • Generated transgenic mice carrying the Emx1-cre insertion.
  • Assessed beta-galactosidase activity and Cre protein presence and function in the cerebral cortex.

    • Main Results:

    • Emx1-specific expression of lacZ and cre genes was achieved in transgenic mice.
    • Beta-galactosidase activity distribution mirrored endogenous Emx1 expression patterns.
    • Cre protein was detected in the cerebral cortex and demonstrated loxP-specific recombination in vitro.

    Conclusions:

    • The developed Emx1-cre transgenic mouse line exhibits specific expression in the cerebral cortex and hippocampus.
    • This model provides a valuable tool for investigating gene function, mutation effects, and overexpression impacts on brain development and plasticity.
    • The Emx1 promoter's restricted pattern facilitates targeted genetic manipulation in key brain regions.