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Cloning and expression of CIS6, chromosome assignment to 3p22 and 2p21 by in situ hybridization

F Magrangeas1, F Apiou, S Denis

  • 1INSERM U463, Institut de Biologie, Nantes, France.

Cytogenetics and Cell Genetics
|April 25, 2000
PubMed

Insights

Researchers identified CIS6, a new member of the suppressor of cytokines signaling (SOCS) family. This protein may negatively regulate the JAK signaling pathway, crucial in cellular communication and immune responses.

Area of Science:

  • Molecular Biology
  • Signal Transduction
  • Genetics

Background:

  • The suppressor of cytokines signaling (SOCS) / cytokine-inducible SH2 protein (CIS) family are negative regulators of JAK signaling.
  • Identifying novel members of this family is crucial for understanding signal transduction regulation.

Purpose of the Study:

  • To identify novel members of the SOCS/CIS family.
  • To characterize the human homologue of SOCS-5, designated CIS6.

Main Methods:

  • Database searching using a consensus amino acid sequence of the SH2 domain.
  • Isolation of cDNA coding for CIS6.
  • Northern blot analysis for mRNA expression.
  • In situ hybridization for gene mapping.

Main Results:

  • CIS6, the human homologue of SOCS-5, was identified.
  • CIS6 mRNA is expressed in heart, muscle, spleen, thymus, and myeloma cell lines.
  • The CIS6 gene was mapped to human chromosome bands 2p21 and 3p22.

Conclusions:

  • CIS6 is a novel member of the CIS family of proteins.
  • CIS6 is structurally related to other CIS proteins.
  • CIS6 has the potential to act as a negative regulator of signal transduction pathways, particularly JAK signaling.

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