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Cloning and expression of CIS6, chromosome assignment to 3p22 and 2p21 by in situ hybridization
F Magrangeas1, F Apiou, S Denis
1INSERM U463, Institut de Biologie, Nantes, France.
Abstract:
A family of negative regulators of JAK signaling pathway referred to as suppressor of cytokines signaling (SOCS) or cytokine-inducible SH2 protein (CIS) has been recently identified. In order to find additional members of this family, we have used a consensus amino acid sequence contained in the well-conserved central SH2 domain to search DNA databases. We isolated cDNA coding for the human homologue of SOCS-5, referred to as CIS6. Northern blot analysis revealed CIS6 mRNA expression in various tissues such as heart, muscle, spleen, and thymus and in all myeloma cell lines examined. The gene was assigned to human chromosome bands 2p21 and 3p22 by in situ hybridization. CIS6 is structurally related to other members of the CIS family and therefore could act as a negative regulator of signal transduction.
Insights
Researchers identified CIS6, a new member of the suppressor of cytokines signaling (SOCS) family. This protein may negatively regulate the JAK signaling pathway, crucial in cellular communication and immune responses.
Area of Science:
- Molecular Biology
- Signal Transduction
- Genetics
Background:
- The suppressor of cytokines signaling (SOCS) / cytokine-inducible SH2 protein (CIS) family are negative regulators of JAK signaling.
- Identifying novel members of this family is crucial for understanding signal transduction regulation.
Purpose of the Study:
- To identify novel members of the SOCS/CIS family.
- To characterize the human homologue of SOCS-5, designated CIS6.
Main Methods:
- Database searching using a consensus amino acid sequence of the SH2 domain.
- Isolation of cDNA coding for CIS6.
- Northern blot analysis for mRNA expression.
- In situ hybridization for gene mapping.
Main Results:
- CIS6, the human homologue of SOCS-5, was identified.
- CIS6 mRNA is expressed in heart, muscle, spleen, thymus, and myeloma cell lines.
- The CIS6 gene was mapped to human chromosome bands 2p21 and 3p22.
Conclusions:
- CIS6 is a novel member of the CIS family of proteins.
- CIS6 is structurally related to other CIS proteins.
- CIS6 has the potential to act as a negative regulator of signal transduction pathways, particularly JAK signaling.