Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transport of peptide-MHC class II complexes in developing dendritic cells.

S J Turley1, K Inaba, W S Garrett

  • 1Department of Cell Biology and Section of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520-8002, USA.

Science (New York, N.Y.)
|April 25, 2000
PubMed
Summary

Dendritic cells (DCs) transport processed antigens on Major Histocompatibility Complex class II (MHC II) molecules. These peptide-MHC II complexes accumulate in specialized vesicles for efficient T cell receptor (TCR) signaling upon cell surface presentation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026
Same author

Intratumoral heterogeneity and immunotherapy resistance: clinical implications.

Annals of oncology : official journal of the European Society for Medical Oncology·2025
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025
Same author

Does the 1:1:1 Transfusion Ratio Mortality Benefit Hold True in Ultra-massive Transfusion? A Study From the Product (Patient Related Outcomes During Ultra-massive transfusion multi-Center Trial) Consortium.

The American surgeon·2025
Same author

Validation of the ^{10}Be Ground-State Molecular Structure Using ^{10}Be(p,pα)^{6}He Triple Differential Reaction Cross-Section Measurements.

Physical review letters·2023
Same author

Abstracts of presentations to the Annual Meetings of the Canadian Society of Colon and Rectal Surgeons Canadian Association of General Surgeons Canadian Association of Thoracic Surgeons: Canadian Surgery Forum, London, Ont., Sept. 19 to 22, 2002.

Canadian journal of surgery. Journal canadien de chirurgie·2023

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Major histocompatibility complex class II (MHC II) molecules present peptides to T cell receptors (TCRs).
  • Dendritic cells (DCs) process antigens in the endocytic pathway to form peptide-MHC II complexes.
  • DC maturation triggers the transport of these complexes to the cell surface.

Purpose of the Study:

  • To investigate the transport mechanism of peptide-MHC II complexes in dendritic cells.
  • To characterize the composition and trafficking of MHC II-containing vesicles.
  • To understand how DCs present peptide-MHC II complexes for T cell activation.

Main Methods:

  • Analysis of antigen processing and presentation in immature and mature DCs.
  • Microscopy and biochemical assays to identify vesicle contents.

Related Experiment Videos

  • Tracking of MHC II-positive vesicles and their migration to the plasma membrane.

    • Main Results:

    • Immature DCs sequester antigens in lysosomes, processing them into peptide-MHC II complexes upon maturation.
    • Mature DCs utilize distinct, non-lysosomal vesicles for MHC II transport, excluding lysosomal enzymes.
    • These vesicles contain MHC I and B7 costimulatory molecules, concentrating TCR ligands at the cell surface.

    Conclusions:

    • Dendritic cells employ a specialized vesicular transport pathway to deliver peptide-MHC II complexes to the plasma membrane.
    • This mechanism ensures selective concentration of TCR ligands and costimulatory molecules for optimal T cell interaction.
    • The findings provide insights into the regulation of adaptive immune responses by antigen-presenting cells.