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Related Experiment Videos

Vitis vinifera must varietal authentication using microsatellite DNA analysis (SSR).

M A Faria1, R Magalhães, M A Ferreira

  • 1CEQUP/Serviço de Bromatologia, Faculdade de Farmácia, Universidade do Porto, Rua de Aníbal Cunha, 4050-047 Porto, Portugal. bromato@ff.up.pt

Journal of Agricultural and Food Chemistry
|April 25, 2000
PubMed
Summary

This study presents a DNA method for authenticating Vitis vinifera grape must. Microsatellite analysis successfully identified grape varieties and their mixtures, aiding in port wine authentication.

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Area of Science:

  • Genetics
  • Viticulture
  • Food Science

Background:

  • Grape must authentication is crucial for wine integrity, especially for protected designations like port wine.
  • Accurate varietal identification ensures compliance with origin and quality standards.

Purpose of the Study:

  • To develop and validate a microsatellite DNA-based method for authenticating Vitis vinifera grape must.
  • To assess the method's efficacy in distinguishing between key port wine grape cultivars and their mixtures.

Main Methods:

  • Utilized four established microsatellite loci to genotype five major port wine grape cultivars (Tinta Roriz, Tinto Cão, Touriga Francesa, Touriga Nacional, Tinta Barroca).
  • Analyzed DNA profiles of individual varietal musts and 26 distinct must mixtures derived from these cultivars.

Related Experiment Videos

  • Compared DNA profiles from grape leaves and their corresponding musts.

    • Main Results:

    • No discrepancies were observed between grape leaf and varietal must DNA profiles.
    • All analyzed must mixtures exhibited expected microsatellite band profiles, reflecting the combined varietal components.
    • The four-locus microsatellite method successfully discriminated between 8 out of the 26 tested must mixtures.

    Conclusions:

    • Microsatellite DNA profiling provides a reliable method for Vitis vinifera grape must authentication.
    • The developed method can differentiate between key port wine grape varieties and their mixtures, supporting quality control.