Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Trauma increases extrahepatic arginase activity.

J B Ochoa1, A C Bernard, S K Mistry

  • 1Department of Surgery, University of Kentucky, Lexington 40536, USA.

Surgery
|April 25, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cerebral homeostasis and orthostatic responses in residents of the highest city in the world.

Scientific reports·2024
Same author

[Congenital heart disease : fistula from circumflex artery to coronary sinus].

Revue medicale de Liege·2019
Same author

Tube Feed Necrosis after Major Gastrointestinal Oncologic Surgery: Institutional Lessons and a Review of the Literature.

Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract·2017
Same author

Risk factors of overweight and obesity in childhood and adolescence in South Asian countries: a systematic review of the evidence.

Public health·2015
Same author

TGF-β signaling alters the pattern of liver tumorigenesis induced by Pten inactivation.

Oncogene·2014
Same author

Stretchable liquid-crystal blue-phase gels.

Nature materials·2014

Trauma significantly increases arginase activity in immune cells, particularly splenic macrophages. This elevated arginase may support tissue repair or regulate nitric oxide production following injury.

Area of Science:

  • Immunology
  • Physiology
  • Biochemistry

Background:

  • Arginase activity, primarily in the liver, is also found in extrahepatic tissues like macrophages.
  • In immune cells, arginase plays roles in wound healing, proliferation, and nitric oxide (NO) regulation.
  • Trauma increases factors that upregulate arginase, suggesting a potential increase in immune cell arginase activity post-trauma.

Purpose of the Study:

  • To test the hypothesis that arginase activity increases in immune cells following surgical trauma.
  • To investigate the temporal changes in arginase activity and expression after trauma.
  • To examine the relationship between arginase activity, inducible nitric oxide synthase (iNOS), and NO metabolites post-trauma.

Main Methods:

  • A surgical trauma model was established in C3H/HeN mice via exploratory laparotomy.

Related Experiment Videos

  • Tissue arginase activity and arginase I protein expression were measured.
  • Endotoxin was used to stimulate arginase activity and expression as a control; iNOS expression and NO metabolites were also assessed.

    • Main Results:

    • Surgical trauma significantly increased arginase activity in splenic and renal tissues, with splenic macrophages showing a tenfold increase.
    • Arginase activity remained elevated for up to 4 days post-trauma, while arginase I expression was upregulated in splenic and renal tissues.
    • Despite increased iNOS, circulating nitric oxide metabolites decreased after trauma, indicating complex NO regulation.

    Conclusions:

    • Extrahepatic arginase expression and activity are elevated following trauma.
    • Increased arginase may contribute to cellular proliferation and tissue repair processes.
    • Arginase upregulation after trauma may play a regulatory role in nitric oxide production.