Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lessons from sequenced genomes. Overlapping genes in Methanococcus jannaschii?

M Szymanski1, J Barciszewski

  • 1Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Poznan, Poland.

IUBMB Life
|April 25, 2000
PubMed
Summary

Overlapping genes in the Methanococcus jannaschii genome were identified. A transfer RNA (tRNA) sequence within an open reading frame (ORF) suggests alternative translation start sites, impacting gene expression analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modification of χ_{c1}(3872) and ψ(2S) Production in pPb Collisions at sqrt[s_{NN}]=8.16  TeV.

Physical review letters·2024
Same author

Amplitude Analysis of the B^{0}→K^{*0}μ^{+}μ^{-} Decay.

Physical review letters·2024
Same author

Fraction of χ_{c} Decays in Prompt J/ψ Production Measured in pPb Collisions at sqrt[s_{NN}]=8.16  TeV.

Physical review letters·2024
Same author

Enhanced Production of Λ_{b}^{0} Baryons in High-Multiplicity pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2024
Same author

Observation of Cabibbo-Suppressed Two-Body Hadronic Decays and Precision Mass Measurement of the Ω_{c}^{0} Baryon.

Physical review letters·2024
Same author

Improved Measurement of CP Violation Parameters in B_{s}^{0}→J/ψK^{+}K^{-} Decays in the Vicinity of the ϕ(1020) Resonance.

Physical review letters·2024

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • The Methanococcus jannaschii genome presents complex gene organization.
  • Overlapping genes and alternative translation initiation sites pose challenges in accurate gene annotation.

Purpose of the Study:

  • To investigate the implications of a tRNA(Ser) sequence within a Methanococcus jannaschii open reading frame (ORF).
  • To re-evaluate the assigned initiation codon for the identified ORF and explore alternative start sites.

Main Methods:

  • Bioinformatic analysis of the Methanococcus jannaschii genome sequence.
  • Identification of open reading frames (ORFs) and potential translation initiation sites.
  • Analysis of regulatory sequences, including Shine-Dalgarno sequences.

Related Experiment Videos

Main Results:

  • A transfer RNA (tRNA) sequence was found within an ORF, questioning the initial translation start site.
  • Two alternative AUG initiation codons were identified downstream of the original TTG start site.
  • Only one of the alternative AUG codons is associated with a Shine-Dalgarno sequence.

Conclusions:

  • The presence of tRNA(Ser) necessitates a re-evaluation of ORF initiation codon assignment in M. jannaschii.
  • Alternative AUG start codons, particularly those with Shine-Dalgarno sequences, are likely functional translation initiation sites.
  • This finding impacts the understanding of gene expression and regulation in M. jannaschii.