Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Histone deacetylases specifically down-regulate p53-dependent gene activation.

L J Juan1, W J Shia, M H Chen

  • 1National Health Research Institutes, 128 Yen-Chiu-Yuan Road, Sec 2, Taipei 115, Taiwan.

The Journal of Biological Chemistry
|April 25, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: PPARγ is essential for protection against nonalcoholic steatohepatitis.

Gene therapy·2025
Same author

Deliberating performance targets workshop: Potential paths for emerging PM<sub>2.5</sub> and O<sub>3</sub> air sensor progress.

Atmospheric Environment: X·2021
Same author

Hyper-viscoelastic mechanical behavior of cranial pia mater in tension.

Clinical biomechanics (Bristol, Avon)·2020
Same author

Prognosis and clinicopathologic features in patients with gastric stump cancer after curative surgery.

Current oncology (Toronto, Ont.)·2020
Same author

PARP-1 Involvement in Autophagy and Their Roles in Apoptosis of Vascular Smooth Muscle Cells under Oxidative Stress.

Folia biologica·2018
Same author

Identification of novel and conserved microRNA and their expression in the gray mouse lemur, Microcebus murinus, a primate capable of daily torpor.

Gene·2018

Histone deacetylases (HDACs) regulate the tumor suppressor p53 by removing acetyl groups, which reduces p53

Area of Science:

  • Molecular Biology
  • Cancer Biology
  • Epigenetics

Background:

  • p53 is a critical tumor suppressor gene frequently mutated in cancer.
  • p53 activity is regulated by posttranslational modifications, including acetylation.
  • Acetylation generally enhances p53 activity, promoting cell cycle arrest or apoptosis.

Purpose of the Study:

  • To investigate the role of mammalian histone deacetylases (HDACs) in regulating p53 function.
  • To determine if HDACs can down-regulate p53 activity through deacetylation.
  • To elucidate the interaction between p53 and HDACs.

Main Methods:

  • Co-expression of HDACs (1, 2, and 3) with p53.
  • Assays to measure p53 acetylation levels and transcriptional activity.

Related Experiment Videos

  • GST pull-down and immunoprecipitation assays to assess protein interactions.
  • In vitro deacetylation assays using p53 peptides and HDAC1.

    • Main Results:

    • HDAC1, HDAC2, and HDAC3 were found to down-regulate p53 function in a dosage-dependent manner.
    • HDAC-mediated down-regulation of p53 requires deacetylase activity and targets specific acetylation sites.
    • p53 directly interacts with HDAC1 both in vitro and in vivo.
    • HDAC1 deacetylated p53 in vitro and reduced p53 acetylation and transcriptional activity in vivo.
    • HDACs reduced p53's activation of the BAX promoter.

    Conclusions:

    • Mammalian HDACs, particularly HDAC1, can deacetylate and inhibit p53 activity.
    • p53 deacetylation by HDACs is a mechanism controlling p53's physiological function.
    • These findings highlight a novel regulatory pathway for the tumor suppressor p53.