Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Slap negatively regulates Src mitogenic function but does not revert Src-induced cell morphology changes.

G Manes1, P Bello, S Roche

  • 1Centre de Recherche de Biochimie Macromoléculaire, Centre National de la Recherche Scientifique UPR-1086, 34293 Montpellier, France.

Molecular and Cellular Biology
|April 25, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Can clinicians predict individual patient outcomes in neuroendocrine tumors treated with [177Lu]Lu-DOTATATE?

The oncologist·2026
Same author

Is it time to introduce health coaching in inflammatory bowel disease management?

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·2025
Same author

The TL1A inhibitors in IBD: what's in the pot?

Expert review of gastroenterology & hepatology·2025
Same author

Invited review: Qualitative social and human science research focusing on actors in and around dairy farming.

Journal of dairy science·2024
Same author

Invited review: Qualitative research in dairy science-A narrative review.

Journal of dairy science·2023
Same author

Calf management and welfare in the Canadian and US dairy industries: Where do we go from here?

Journal of dairy science·2023
Same journal

Aberrant Expression of miR-25-3p/EZH2 Is Involved in T Cell Activation in Aplastic Anemia.

Molecular and cellular biology·2026
Same journal

Characterization of the m<sup>6</sup>A Epitranscriptome in Fibroblast Senescence.

Molecular and cellular biology·2026
Same journal

Insights into FACT in Cancers with Targeted Therapeutic Implications.

Molecular and cellular biology·2026
Same journal

Human lncRNA, hLinfRNA7 (IDO1-AS) Regulates Cytokine Expression, Tryptophan Catabolism, and Inflammatory Response in Macrophage.

Molecular and cellular biology·2026
Same journal

mTORC1-Dependent Regulation of the CCL24-CCR3 Axis Controls Granuloma Formation and Maintenance in Sarcoidosis.

Molecular and cellular biology·2026
Same journal

The Novel Compound SIC-19 Triggers CUL4B-Mediated Ubiquitination and Degradation of SIK2.

Molecular and cellular biology·2026
See all related articles

Src-like adapter protein (Slap) negatively regulates cell growth by inhibiting Src kinase activity. While Slap inhibits DNA synthesis, its distinct SH3 domain prevents it from regulating all Src functions, including cell transformation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Signal Transduction

Background:

  • Src-like adapter protein (Slap) is a novel protein regulating mitogenesis.
  • Slap shares structural similarities with Src, possessing SH3 and SH2 domains.
  • Its role as a potential antagonist of Src functions requires investigation.

Purpose of the Study:

  • To determine if Slap antagonizes all Src functions.
  • To elucidate the specific domains and mechanisms by which Slap regulates Src.
  • To investigate Slap's role in platelet-derived growth factor (PDGF)-induced signaling and cell transformation.

Main Methods:

  • Coexpression of Slap and Src in Cos7 cells.
  • Microinjection of Slap constructs into NIH 3T3 fibroblasts.

Related Experiment Videos

  • Analysis of DNA synthesis and cell transformation.
  • Creation and testing of chimeric Slap/Src constructs.
  • In vitro binding assays for SH3 domain interactions.

    • Main Results:

    • Slap inhibits PDGF-induced DNA synthesis in a noncompetitive manner via its C terminus.
    • Slap does not inhibit Src-induced cell transformation, specifically actin stress fiber depolymerization.
    • Distinct Slap SH3 binding specificity prevents interaction with key Src SH3 partners.
    • Slap acts as a negative regulator of Src mitogenesis through its SH2 and C-terminal domains.

    Conclusions:

    • Slap is a specific negative regulator of Src-mediated mitogenesis.
    • Its inhibitory function involves the SH2 and C-terminal domains.
    • The unique SH3 domain of Slap limits its regulatory capacity to specific Src functions.